JIS Z 2801/ISO 22196 standard was selected as the test procedure. Prior to testing, all the tested samples were cut to 50 x 50mm pieces and soaked for 1 minute in 70% ethanol, which was then dried in an oven at 45°C for 4 hours. The samples of the tested materials were inoculated with 0.1ml of microorganism culture and covered with a film/glass cover to prevent evaporation. The samples were then incubated at 29°C to 37°C according to the microbe’s preferred growing conditions. Viable organisms were recovered by steeping the tested sample in a phosphate buffer and then serially diluting the fluid. Four separate identical samples of 0.1ml of each concentration were then dispersed on Petri dishes containing Nutrient Agar matched to the type of bacteria, then incubated for 24/48 hours at 29°C to 37°C. Colonies were then counted and the concentration was calculated, the result reported is the average. A control (no antimicrobial additive) was run for each microorganism tested (included are some of the different strains of bacteria and Fungi tested).